Wayne State University

Animal Hazardous Agent Forms

Principal Investigators who conduct animal experiments requiring Institutional Animal Care and Use Committee (IACUC) approval must also obtain the appropriate safety approval when work involves the use of hazardous materials.

The Office of Environmental Health and Safety (OEHS) will assist you in preparing the appropriate application documents and safety forms to obtain approval for your use of these agents.

Infectious agents & biological hazards requiring IACUC, OEHS and IBC Approval

  • The Animal Hazardous Agents Form (AHAF) specific to the agent and host species should be drafted and reviewed for appropriate safety practices and procedures connected with experimentally infected animals. A copy of the AHAF must be placed on the door of a room housing animals exposed to hazardous substances.
  • Culturing, propagating, or preparation of infectious agents in the laboratory requires Institutional Biosafety Committee (IBC) review and approval of written safety procedures. Animals exposed to potentially infectious microorganisms are housed under special conditions. Additional housing requirements, entry precautions, and signage are also needed.

Toxic chemicals & hazardous drugs requiring IACUC and OEHS approval

  • The Animal Hazardous Agents Form (AHAF)  must be placed on the door of a room housing animals when there is a possibility for the administered hazardous agents or metabolites to be excreted by the animal.
  • The potential for an exposure with significant risk is greatest during the preparation of stock solutions of carcinogens, reproductive toxins, chemotherapeutic drugs and other highly toxic agents where chemicals are manipulated in their pure or concentrated state. Lab safety procedures should be written and submitted to OEHS for review.

Radioisotopes requiring IACUC, OEHS and RSC approval

  • If you intend to inject radioisotopes into laboratory animals, this work will require Radiation Safety Committee (RSC) review and approval of written safety procedures prior to use of these materials.

Animal hazardous agents forms (AHAF)

A separate AHAF must be completed for chemical hazards and biological hazards. Provide a copy to the DLAR staff at least one week prior to the exposure of your animals to hazardous agents.

AHAF for Biological Agents (native or recombinant mammalian viruses, bacteria, fungi, human blood, tissue, cell Lines, etc.)

AHAF for Chemical Hazards

Specific animal hazardous agent forms:

Bromodeoxyuridine (BrdU)

Cytotoxins/Chemotherapy Agents

Estrogen Pellets

Complete Freund's Adjuvant

Manganese Chloride

Select Agent Toxin Autoclave Deactivation *

Select Agent Toxin Chemical Deactivation *

Streptozotocin (STZ)

Streptozotocin (STZ) - Metabolic Cages

Toxic Agent Autoclave Deactivation

Toxic Agent Chemical Deactivatiion

* Select Agent Toxins include: Abrin, Botulinum neurotoxins, Botulinum neurotoxin producing species of Clostridium, Clostridium perfringens epsilon toxin, Conotoxins, Ricin, Saxitoxin, Shigatoxin, Staphylococcal enterotoxins, T-2 toxin, Tetrodotoxin. For a complete list of Select Agents, click here.