Standard Operating Procedures (SOPs) and other attachments for eProtocol submissions

For applicants using the eProtocol format, some documents are still to be completed in the Word format.  These documents must then be added to the eProtocol submission as attachments. 

General SOPs:

In vivo SOP:  Please complete and attach this SOP if your proposed research involves the use of animal models.

In vitro SOP:  Please complete and attach this SOP for all work to be performed in vitro.

• For assistance with SOPs: Preparing a Standard Operating Procedure - Guidance from the IBC

Specific SOPs:

Transgenic Drosophila are covered by the NIH Guidelines and IBC approval is required for this type of research activity.  Through collaboration with the WSU Drosophila research community, the following SOP and guidance document were developed.

Drosophila SOP:  Please complete this SOP if you are working with transgenic Drosophila

• For assistance with Drosophila SOPs: Guidance from the IBC

Transgenic Zebrafish are covered by the NIH Guidelines and IBC approval is required for use of this animal model at WSU.  Through collaboration with the WSU Zebrafish research community, the following SOP was developed.  Please note that the waste disposal sections have been completed based on best practice recommendations identified by WSU OEHS.

Transgenic Zebrafish SOP:  Please complete this SOP if you are working with transgenic Zebrafish

Transgenic strains of Caenorhabditis elegans are covered by the NIH Guidelines and IBC approval is required for use of this animal model at WSU.  Through collaboration with the WSU C. elegans research community, the following SOP was developed. 

Transgenic C. elegans SOP:  Please complete this SOP if you are working with transgenic strains of C. elegans

Select Agent, Select Agent Toxin, and Toxins with high acute toxicity SOPs and forms:

IBC approval is required for the possession, use, and transfer of unfractionated mixtures and purified preparations of biological toxins having high acute toxicity.  This includes all toxins on the Select Agent list.  

High acute toxicity is indicated by toxins with a LD50 of less than, or equivalent to, 100 nanograms/kg body weight.  

IBC approval must be obtained prior to this material being brought onto WSU Campus.

• Select Agent/Toxin SOP
• Select Agent Toxin User Log - Complete the top portion of this form and maintain this user log for all Select Agent Toxins
• Select Agent Toxin Destruction Form 
• Diphtheria Toxin:  This SOP alone is required if you are working with Diphtheria toxin
• Toxin User Log:  Complete and maintain this user log for all non-select agent toxins
• Toxin lab inspection checklist

Inactivated High Risk Agents (Select Agents, Select Agent Toxins, Risk Group 3 or 4 pathogens) 

• Research groups or core facilities that plan on receiving inactivated high risk biological agents must obtain prior approval from the WSU IBC.  Please complete and attach the SOP to the e-Protocol application.

US Army sponsored work with cadaveric material

• All research activity that is being supported by grants from the US Army and involve the use of cadaveric material must obtain approval from the WSU IBC.  Please complete and attach the SOP to the e-Protocol application.  Please contact the WSU Biosafety Officer before beginning the application.

Other e-protocol attachments:

Lentiviral Vector Protocols:

• Lentiviral Vector Exposure Response plan:  (only page 3 needs to be submitted) If you are working with multiple lentiviral vectors then please consider categorizing the constructs based on risk and completing one form for each risk group identified

Use of biohazardous agents in animals:

• Animal Hazardous Agent Form II (Biosafety) - This should be specific to the agent and host species.  It will be reviewed by the IBC for appropriate safety practices and procedures connected with experimentally infected animals. A copy of the AHAF II must be placed on the door of a room housing animals exposed to hazardous substances.  Animals exposed to potentially infectious microorganisms are housed under special conditions. Additional housing requirements, entry precautions, and signage are also needed.