The National Institutes of Health (NIH) published a report on the importance of enhancing rigor, transparency, and translatability in animal research in the summer of 2021. Since then, aspects of this report have been adopted by the Association for Accreditation and Assessment of Laboratory Animal Care, International (AAALAC) in July 2022 and most recently by NIH in a published federal notice in February 2023. The NIH Report listed two tools that can enhance rigor of animal research – the ARRIVE 2.0 Guidelines and the Experimental Design Assistant. Both AAALAC and NIH strongly recommend animal researchers use the ARRIVE 2.0 Guidelines in IACUC protocols and publications resulting from NIH funding.  AAALAC also recommends animal researchers utilize the ARRIVE 2.0 Guidelines and the Experimental Design Assistant when developing research protocols. The IACUC Office has created a presentation describing the ARRIVE 2.0 Guidelines, as well as the Experimental Design Assistant. The presentation can be accessed here or by contacting the IACUC Office directly at IACUC@wayne.edu. We hope you find it useful.